abstract
Myeloid-derived suppressor cells (MDSCs) are a population of heterogeneous cells originating from the myeloid lineage whose further characterization could lead to the development of an antitumor drug due to MDSC’s heavy role in cancer cell growth and metastasis. Research into MDSCs is limited due to their severe heterogeneity and inability to be produced in large quantities in vivo. In order to study these enigmatic cells, MDSCs were differentiated in vitro. In addition, a cross strain analysis was performed, and an evaluation of interleukin-6 (IL-6) was performed. We determined that MDSCs can be differentiated in vitro, producing sufficient amounts of M-MDSCs and G-MDSCs that are physiologically and functionally similar to those differentiated in vivo. MDSCs differentiation can occur between Black-6 and BALB/c 4T1 cells. In addition, IL-6 is heavily involved in MDSC differentiation. In the future, studies on further characterization should occur. Namely, research into MDSCs with a Ly6C+Ly6G+ phenotype ought to be studied.
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research questionsa.) Can MDSCs be differentiated in vitro from BALB/c bone marrow derived stem cells?
b.) Can C57BL-6 bone marrow derived stem cells be differentiated using BALB/c 4T1 conditioned medium? c.) Is interleukin-6 the primary cytokine that causes MDSC differentiation? |
hypothesesa.) MDSC differentiation will occur when bone marrow derived stem cells are grown in 4T1 conditioned medium.
b.) C57BL-6 bone marrow derived stem cells grown in BALB/c 4T1 conditioned medium will differentiate into MDSCs. c.) Interleukin-6 is the primary cytokine that causes MDSC differentiation. |
about the authorGrace Nunnelee is a senior at Ridgeview Classical Schools in Fort Collins, CO. She is a member of National Science Bowl and Hoplite Helpers, her school's philanthropy club. She is an aspiring biologist with an interest in genetics and inflammation. For fun, Grace likes to hike and go on long walks with her dogs.
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about the programThe Frontiers of Science Institute is a six week long intensive research camp for high school seniors and juniors excelling in STEM. The program consists of research, lectures, classes, and industry tours.
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